Discovery of nitrogenous derivatives of dihydromyricetin in cell culture medium

Dihydromyricetin (DMY) is a health-beneficial flavanonol in the family of flavonoids, extracted from Ampelopsis grossedentata (Hand.-Mazz.) W. Wang. When investigating stability DMY Dulbecco's modified Eagle's medium (DMEM) solution at 37°C by LC-MS/MS, four nitrogenous derivatives were found except for previously known dimers, oxidized products, and ring-cleavage products DMY. Compound 1 (N-DMY) was identified to be 4′-amino (NH2)-DMY based on MS NMR data. The formation mechanism N-DMY thought include two steps. First, pyrogallol moiety ring B prone quinone. Second, quinone might further participate Strecker degradation amino acids DMEM form corresponding aldehydes. Correspondingly, compounds 2–4 formed reaction with quinones oxidation dimers products. Consistent mechanism, it observed that flavonoids catechol or readily their during incubation 37°C, which because are easily quinones. In addition, may have potential metabolism vitro vivo experiments. Therefore, flavonoid play significant role actual effects need investigated future.